Nancy

John Tarpley wrote a nice article in the Journal of Histotechnology
about undecalcifed bone sections and the tape transfer system.  I have
minimal experience but I found that the coated slides with the least
amount of adhesive worked the best (the 1X).  I would only expose the
slides once to the UV light and gently remove the tape starting at one
corner.  Patsy helped me out when I was first doing this.  I remember
her setting the angle of the knife holder different and I can't recall
what that was or what the temp was.  She will probably respond.  When I
was cutting human cortical bone I did get a few fracture marks, but I
think that this artifact is difficult to remove.  A nice sharp tungsten
carbide knife works also and when sectioning an even smooth motion is
helpful.  

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lowen,
Nancy
Sent: Thursday, June 02, 2005 9:34 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Info on Cryojane

If anyone is using the Cryojane tape transfer system for cutting
undecalcified bone for frozen sections, we would appreciate your imput.
What temperature do you cut bone at and does the tape transfer the
complete
section of bone to the slide?

We are getting shattering of the bone on most sections and are really
having
some problems with the bubbles not rolling out. 

We are still in the testing process, but we would love to have any tips
from
anyone who has more experience on how to obtain the best sections.
Should
we expect to see totally intact sections when cutting bone?  Also, what
freezing method have you found to be the best..Thanks in advance

Nancy

 

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