RE: [Histonet] Dendritic Cells in Intestine

From:Kim Merriam

Julia, Gayle,
I am not sure exactly what the researcher is looking for, she just asked me if I knew of a stain that would specifically identify these cells (FFPE mouse tissue).  I do have a DVM-pathologist here that will be reading the slides.  I had hoped there was some type of special stain that I could do (rather than going about antibody validation for just one slide).  The researcher will need to check into this.  It looks like we will probably need to do IHC on this one.  Thanks for your input.

Julia Dahl  wrote:
Kim -

There are two main populations of dendritic cells in the intestine - 
dendritic cells of macrophage lineage and dendritic cells of myofibroblast 
lineage. These populations serve some overlapping, but some distinct 
functions. Which types of cells/functions are you wanting to identify? 
That will specify the type of antibody that you will want to use.

Do you have a pathologist on staff that understands the difference between 
these cells? If not, I can provide a more in depth consultation than the 

Julia Dahl, M.D.
Mosaic Gastrointestinal Research Consortium
Author: COLON in Histology for Pathologists, 3rd Edition; In press

>From: Kim Merriam 
>To: Histonet 
>Subject: [Histonet] Dendritic Cells in Intestine
>Date: Thu, 19 May 2005 06:22:46 -0700 (PDT)
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>Hello everyone,
>Does anyone know of a special stain that will identify dendritic cells in 
>the intestine?
>Kim Merriam
>Cambridge, MA
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