RE: [Histonet] CD44 v4 and v7

From:"Young Kwun"

Hi Heather,
I am not sure about CD44 v4 & v7, but I tried CD44 v6 antibody (dil. 1/500)
from R&D Systems (Cat No.BRA13). I obtained good results with HIER with EDTA
(pH 8) or Citrate buffer.



Young Kwun
Senior Hospital Scientist
Dept of Anatomical Pathology
Concord Hospital
Concord NSW 2139
Phone:61-2-9767 6075
Fax:61-2-9767 8427


-----Original Message-----
[] On Behalf Of Mcleod
Sent: Tuesday, 24 May 2005 11:44 PM
Subject: [Histonet] CD44 v4 and v7

Dear Histonetters

A colleague is having trouble with these 2 antibodies.  She has tried
hier at ph 6
and 8, enzyme ar and no ar without any success.  Her problem is specific
amidst lots of non-specific staining and when the dilution is increased
the desired signal is lost and she is left with all of the nss.
If anybody has successfully worked with these antibodies; any advice /
help would
be appreciated.
She is using Novocastra CD44 v4 and 
Serotec CD44 v7 (MCA1731)

Many many thanks.


Histonet mailing list

"This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service."

Histonet mailing list

<< Previous Message | Next Message >>