RE: [Histonet] BCIP/NBT ready-to-use

From:"Majdic Gregor"

We are using Roche BCIP/NBT readyy to usee tablets for DIG - In situ hybridisation on both brain and testes and I think they work fine. If you have other conditions optimized, tehy give very little background.
Possunt quia posse videntur
Gregor Majdic
Head of Center for Animal Genomics
Veterinary faculty, University of Ljubljana
Gerbiceva 60
SI-1000 Ljubljana, Slovenia
Phone: +386 1 4779210
Fax: +386 1 2832243


From: on behalf of Johnson, Teri
Sent: čet 16.6.2005 0:22
To: Histonet
Subject: [Histonet] BCIP/NBT ready-to-use

We are currently using a substrate system which has two separate
components for BCIP and NBT, and then have to combine them just before
use.  I'm looking into alternate systems and have seen several vendors
who offer what appears to be ready-to-use substrate. Is anybody using
them and can you comment on stability with regard to expiration date,
length of use, and lack of problems after the slides have been stained
(I recall a recent thread regarding horrible overstaining after the
slides had been coverslipped).  Thanks muchly!

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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