[Histonet] stain needed to dye external pores

From:Carla M Conway

Hello all,

A colleague has some juvenile trout (4-8 inches long) that have been fixed
in 10% NBF then stored in ethanol. He would like to count the mandibular
pores located on the underside of the lower jaw. Can anyone suggest a stain
or procedure which would make these external pores more visible? They would
prefer to stain the area directly and not process/paraffin embed, etc.. Any
suggestions will be greatly appreciated.

Carla Conway

Histologist
Western Fisheries Research Center
6505 NE 65th St.
Seattle, WA 98115
ph: 206-526-6282 ext 242
fax: 206-526-6654
cmconway@usgs.gov



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