I have been asked to elaborate on my reference to washing tissues to reverse
the effects of formalin fixation iliminating the possible need for AR.
Here goes:
 
 
I found the reference to Washing in a book called Introduction to
Immunocytochemistry 3rd Edition  J.M. Polak and S. Van Noorden pg. 24- 3.8.2
"The simplest form of reversing the effects of formalin is to wash the
tissue well before processing, but this is not usually possible in
histopathology laboratories, where rapid turnowver of specimens is
required."
 
I have not tested this extensively but have done a little, especially on
over fixed samples, the data is yet to be confirmed but I am looking into it
as we speak.  I will keep you all posted.
 
Patsy
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