[Histonet] problem with MMA
I am using MMA/ BMA to embed titanium samples on wich we grew cells . The
polymerisation step is done at -20°C. Thick sections are made with diamond
thread saw and grinded until they reach 50-80 um. Sections are stained with RBS
Rapid bone stain) on a hot plate at 56°C.
Unfortunately i see on the resulting slides a separation between titanium
can anyone help me understanding what happens and how avoid this separation =20
between titanium and cells.
Can it be due to the retraction of BMA/MMA during polymerization ?
Do you think the temperature (-20°C) during polymerization has an effect ?
In some cases we covered thentitanium with a hydroxyapatite layer before
growing cells, but obtained the same results in the end.
thanks in advance.
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