[Histonet] polyoma virus

From:"Van Eyck, Deb"

Hi all,

   A question for any of you out there doing polyoma virus on urine cyto
specs----I am working up Nova Castra's JC/BVK immuno stain for acetone fixed
cell smears. (Works well on thin prep material with a 20n minute cold
acetone post fix).  In your experiences using this or other immuno
procedures are you getting staining in most of the decoy cells as well as
cytoplasmic staining in infected cells and free virus particles?  My results
are very clean and I am seeing some total nuclear staining in some of the
decoy cells, but not all,  some nuclear ring like staining, some cytoplasmic
inclusion like staining and some clumps of what I am thinking are free virus
particles?   Any one out there using SV-40 for cyto specs and polyoma virus?
If so would you please share your protocol with me? thanks very much.

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