[Histonet] erythroid precursor marker for bone marrow, IF
Dear Histonetters,
I'm trying to find an antibody to use for a marker for erythroid
precursors in mouse bone marrow. I'm able to use TER-119 in FFPE
tissue using DAB but when I do immunofluorescence there's too much
background fluorescence. I managed to quench the background
fluorescence by fixing in methacarn and then leaving the sections in
3% H2O2/50% methanol for several hours, but then TER-119 wouldn't
stain the sections (other abs worked so I must have damaged the
antigen). I'm thinking about trying cd235a/glycoophorin A either
clone HIR2 or JC159. Any suggestions?
Thanks,
Sharon
--
Sharon Cooperman
NIH, NICHD, CBMB 301.435-8417
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892
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