[Histonet] colloidal iron
Since my attachment didn't work, I am sending my protocol for Mowry's
colloidal iron as text.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161
Mowry, R.W. (1958) Improved procedure for the staining of acidic
polysaccharides by Muller's colloidal (hydrous) ferric oxide
and its combination with the Feulgen and periodic acid-Schiff
reactions. J. Clin. Invest. -7-: 566-576.
Stock "solution" (actually a colloidal suspension):
6.75 g FeCl3.6H2O
6 ml distilled water
0.4 ml conc. (37%) HCl
Stir until dissolved.
Bring 250 ml distilled water to a boil in a 1 liter container.
Add ferric chloride solution to the boiling water.
15 ml distilled water
5 ml glacial acetic acid
20 ml stock "solution"
15 ml 2% potassium ferrocyanide trihydrate
[0.3 g K4Fe(CN)6.H2O in 15 ml H2O]
Make 30 minutes before use.
15 ml 2% solution of conc. (37%) HCl
[0.3 ml 37% HCl plus 14.7 ml H2O]
Mix solutions just before use.
1 N HCl:
27.5 ml H2O
2.5 ml conc. (37%) HCl
0.5 g sodium pyrosulfite = "sodium metabisulfite" = Na2S2O5
100 ml distilled water
0.5 ml conc. (37%) HCl
1. Bring to water.
2. 12% acetic acid, 30 seconds.
3. Colloidal iron, 1 hour.
4. 12% acetic acid, 4 changes, 3 minutes each.
5. Ferrocyanic acid, 20 minutes.
6. Running tap water, 5 minutes.
7. 1 N HCl at 60oC, 10 minutes.
8. Running tap water, 5 minutes.
9. Distilled water, rinse.
10. Schiff's reagent, 10 minutes.
11. Sulfurous acid, 3 changes, 2 minutes each.
12. Running water, 5 minutes.
13. Dehydrate, clear, and mount.
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