[Histonet] Re: stain rack

From:Galina Deyneko

Cindy,
Look VWR catalog, p.750, cat # 25608-868 called Slide holder, 24 place.
Galina Deyneko
Novartis Cambridge

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Today's Topics:

1. Re: Stain Racks (Fred Underwood)
2. Fw: [Histonet] Cryostat Frozen Sectioning Aid (Instrumedics)
3. Info on Cryojane (Lowen, Nancy)
4. different antigens need different methods for antigen
retrieval? (pex)
5. Congo Red/Toludine Blue for Eosinaphils and Mast (Madary, Joseph)


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Message: 1
Date: Thu, 02 Jun 2005 11:58:08 -0400
From: "Fred Underwood" 
Subject: Re: [Histonet] Stain Racks
To: , 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Hi Cindy,

Cardinal Health carries them. The catalog number is s7640 15. Their
phone number is 1.888.444.5440. Price is $216 for a package of 10.

Fred

>>> Cindy DuBois 05/31/05 10:19AM >>>
Does anyone know where I can purchase slide racks for the TissueTek DRS
?
We currently run about 250 slides a day and only have 6 racks (one
which is broken). Sakura is not currently manufacturing them due to a
production problem. 

Any suggestions is greatly appreciated,

Cindy Dubois
Delta Pathology Assoc.

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Message: 2
Date: Thu, 2 Jun 2005 12:23:53 -0400
From: "Instrumedics" 
Subject: Fw: [Histonet] Cryostat Frozen Sectioning Aid
To: "HistoNet Server" 
Message-ID: <01c501c5678f$7caddb00$6401a8c0@INSTRUMEDICS22>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=response


----- Original Message ----- 
From: "Instrumedics" 
To: "Susan Q Wells" 
Sent: Thursday, June 02, 2005 12:15 PM
Subject: Re: [Histonet] Cryostat Frozen Sectioning Aid


> Susan
> The shelf life of the adhesive coated slides is years as long as the 
> slides
> are not exposed to UV light.
>
> Bernice
> Instrumedics
>
>
> ----- Original Message ----- 
> From: "Susan Q Wells" 
> To: 
> Sent: Thursday, June 02, 2005 9:02 AM
> Subject: [Histonet] Cryostat Frozen Sectioning Aid
>
>
>> Anyone know what the shelf life of the adhesive coated slides for the
>> Instrumedics system is?
>> Thanks in advance,
>> Susan Wells
>>
>>
>>
> 





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Message: 3
Date: Thu, 2 Jun 2005 09:33:54 -0700 
From: "Lowen, Nancy" 
Subject: [Histonet] Info on Cryojane
To: "'histonet@lists.utsouthwestern.edu'"

Message-ID:

Content-Type: text/plain

If anyone is using the Cryojane tape transfer system for cutting
undecalcified bone for frozen sections, we would appreciate your imput.
What temperature do you cut bone at and does the tape transfer the complete
section of bone to the slide?

We are getting shattering of the bone on most sections and are really having
some problems with the bubbles not rolling out. 

We are still in the testing process, but we would love to have any tips from
anyone who has more experience on how to obtain the best sections. Should
we expect to see totally intact sections when cutting bone? Also, what
freezing method have you found to be the best..Thanks in advance

Nancy





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Message: 4
Date: Fri, 3 Jun 2005 00:46:45 +0800 (CST)
From: pex 

Subject: [Histonet] different antigens need different methods for
antigen retrieval?
To: Histonet@lists.utsouthwestern.edu
Message-ID: <20050602164645.15307.qmail@web15502.mail.cnb.yahoo.com>
Content-Type: text/plain; charset=gb2312

Hello, all,

I feel confused for immunofluorescence.
I am doing immunofluorescence in vertebra sections. I use the same protocol for immunostaining, but the results are very different, the one is very nice, but the other is weak, I do not know the reasons. Do different antigens need different methods for antigen retrieval? 
Generally, which methods for antigen retrieval in bone sections are better? (Enzyme digestion? or HIER? or both?)
Any suggestions will be helpful for me!

Thank you!





---------------------------------
DO YOU YAHOO!?
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Message: 5
Date: Thu, 2 Jun 2005 12:47:00 -0400
From: "Madary, Joseph" 
Subject: [Histonet] Congo Red/Toludine Blue for Eosinaphils and Mast
To: 
Message-ID:
<746FDB897740814EA52BDDCB5ED1DDBCBB1583@medimmune4.medimmune.com>
Content-Type: text/plain; charset="iso-8859-1"

Anyone hear of a combo CR/TB for Eo's and Mast Cells?



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End of Histonet Digest, Vol 19, Issue 4
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