[Histonet] Oocyte (frog) embedding and sectioning
I'm fishing for any insights into embedding and sectioning Xenopus oocytes.
I have two main problems: Oocytes don't always stay in the block, as if they
are not perfused with paraffin; Sections shatter on the blade or on water
bath before mounting to slide. Have attempted several strategies:
pretreatment with proteinase K to remove membrane, fixing in MEMFA 1h
followed by PBS rinses and PBS storage, fixing in MEMFA overnight then
proceeding directly to dehydration and embedding, using Paraplast and
PolyFin, changing blade angle between 2.5 and 10 degrees.
Stem Cell Institute
University of Minnesota
Express yourself instantly with MSN Messenger! Download today - it's FREE!
Histonet mailing list
<< Previous Message | Next Message >>