[Histonet] Methacarn Solution

From:Mario Muscedere

Hi All,
	I've heard a lot about the plusses of using methacarn for IHC, from 
this board and others.  However I tried twice to make up a solution of 
methacarn (60% absolute MeOH / 30% chloroform / 10% glacial acetic 
acid), but the result is a milky solution filled with small flecks of 
what appear to be white precipitate.  I'm assuming this isn't normal.  
Has anyone had any experience with this sort of problem and know what 
I'm doing wrong?

Mario Muscedere       
Biology Department
Boston University
5 Cummington Street
Boston, MA, 02215 
Office: 409 BRB
Office #: 617-353-6977
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