[Histonet] HER-2 ICC protocol for breast cancer cell lines
I am attempting to stain a couple of breast cancer cell lines for
HER-2. Does anyone have a protocol that I could use? I am trying to
avoid paraffin embedding as I am not set up to do this. I am using
MCF-7, BT474 and MDA-MB-453 cells.
Any help would be appreciated. I am relatively new to
immunostaining, so even if there is no advice for HER-2 in
particular, I would like to hear general staining advice for these
cell lines. I think I have to do an antigen retrieval step, but am
not sure how to modify the FFPE protocols that I have seen.
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