[Histonet] Antibodies for FACS and IHC
Please can anyone help? How are antibodies produced for different
applications different? Is it possible to use antibodies that are
currently being used for IHC, for FACS analysis? We currently have
unconjugated primary antibodies which we use for IHC, could we use a
fluorescent secondary antibody to then use it for FACS. If I wanted to
source a new antibody that could be used for IHC (probably frozen sections)
and FACS - do I need to be sure it's been tested in both applications or
can I make a judgement based on it's performance in just one of the
applications? Is it best to source a fluorescent-conjugated primary? Or
will this mean there won't be enough amplification for the IHC application?
Sorry for all the questions but I'm venturing into new territory!!
Thank you in advance,
Nicola Cragg BSc
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