Re: [Histonet] TRAP on bones
EDTA decalcification is preferred decalcifier. Formic acid is more than
likely killing the enzyme, rendering it unstainable. I have a method from
Hermina Borgerink, if you want it.
At 10:05 AM 5/6/2005, you wrote:
>Good morning all,
>
>I have been trying to stain formic acid/EDTA decalcified formalin fixed
>mouse femurs for osteoclasts with the TRAP stain. I have had little
>success. Does anyone have a protocol that I might be able to try? Any
>suggestions would be greatly appreciated. I have been using the Sigma kit
>#387A thus far.
>
>Thanks,
>Leslie Dixon
>Mayo Clinic Scottsdale Research Histology
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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