Hi Histonetters,
We routinely stain all dog skin lesions with Toluidine Blue and it 
highlights the Mast Cells.
They stain metachromatically and show up with red-purple granules in the 
cytoplasm.
These biopsies are all fixed in 10% Neutral Buffered Formalin.
The method we use is from Gretchen Humason, "Animal tissue Techniques", 3rd 
edition.
It uses a solution of 0.2% Tol Blue in 60% Ethanol for 2 minutes.

   Regards,  Laurie.

At 01:14 PM 05/10/05 -0400, Osborn, Sharon wrote:
>Tom,
>
>Luna's Toluidine Blue Method for Mast Cells is a superior demonstration of
>mast cells using a pink background.  These literally pop up under low power.
>The method is located on pages 311-312 of Luna's Histopathologic Methods and
>color Atlas of special Stains and Tissue Artifacts; 1992.  If you wish a
>copy of the procedure, private email me and I will send to you.
>
>sharon osborn
>DNAX ScheringPlough BioPharma
>Palo Alto, CA
>
>Subject: [Histonet] Giemsa Stain for Mast Cells
>Netters,
>I am looking for a Giemsa stain for Mast Cells that does not have a pinkish
>hue like the Gaffney Giemsa.  We are looking to have the Mast Cells pop out
>at us on low power.  Any suggestions/protocols?
>Thanks,
>Tom
>Tom Galati
>Laboratory Director
>HSRL, Inc.- A GLP Compliant Contract Laboratory
>137 South Main Street
>Woodstock, Virginia  22664
>(540)459-8211
>Fax: (540)459-8217
>tomgalati@hsrl.org
>www.hsrl.org 
>
>
>
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Mr.Laurie Reilly                                              Ph 07 4781 4468
School of Veterinary & Biomedical Science      Fax  07 4779  1526
James Cook University
Townsville  Qld. 
4811                                      laurie.reilly@jcu.edu.au 

Australia.


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