Dear Roxanne,

		I hope this is going to be of assistance to you,  we manufacture all our  microtomes & cryostats from a true 0º to 25º angle on the knife or blade holders. In this way we have set a standard for all our users. I cannot see why others choose arbitrary calibrations and vary them too. You will need to place a place a flat sample onto the cassette or object holder and adjust the knife or blade close to the face of the sample and view from the side  of the edge of the knife or blades facet to make sure you have a 1 to 3º clearance. If this is not a clear description please come back to me and I will email you a sketch or try to place it on "list server images" of the Histonet.org website if someone can inform me how to place it there.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Roxanne Soto [mailto:godsgirlnow@msn.com] 
Sent: 03 May 2005 16:45
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cutting angle


What angles does everyone out there cut at and what kind of blades do you 
use?  I have always cut at 10 with high or low profile blades, but I have 
recently changed jobs and they cut at an angle of 21.  They were told that 
with high profile blades they should use an angle of 21.  I am having a hard 
time cutting at this angle.......
any thoughts?
Roxanne



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