Try contacting the company that makes the microtome, and tell them the brand
of knife you are using. They will tell you the best angle for that microtome
for that knife.

Each brand of microtome is a little bit different. And each brand of blade
is a little bit different. So as you switch from one blade to another (or
same blade but different microtome), the angles have to be adjusted.

There is not one "true" setting, as in "one size fits all". Doesn't work
with microtomes and blades.

Peggy Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto
Sent: Tuesday, May 03, 2005 11:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cutting angle

What angles does everyone out there cut at and what kind of blades do you
use?  I have always cut at 10 with high or low profile blades, but I have
recently changed jobs and they cut at an angle of 21.  They were told that
with high profile blades they should use an angle of 21.  I am having a hard
time cutting at this angle.......
any thoughts?
Roxanne



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