RE: [Histonet] RE: RVU units
Patti
You have raised an interesting question that I feel should be discussed
on Histonet.
There seems to be a national tendency to use RVUs to compare different
procedures and provide some type of standardization. The same process is
being examined in order to compare teaching loads. On the surface it
would appear to be desirable. However there are some drawbacks that I
have seen in discussions of this topic for teaching.
If x number of units are for a lecture given for the first time this
does not take into account the skill of the teacher, the availability of
resources or the different fields of teaching, biochemistry, histology
etc.
I would suggest that the same applies to your question. While it is nice
to be able to compare the RVU of a specific technique that you are
carrying out with that used by other laboratories in the field it may
not be a fair comparison. The actual cost to you depends on several
factors such as, skill of the individual, efficiency of technique and
equipment (which may depend on newness of machine etc), availability of
equipment and resources etc., etc.
This is a complex problem that I feel would be in laboratories interest
to handle at a local level.
Just my opinion.
Barry
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti
Loykasek
Sent: Wednesday, May 11, 2005 11:42 AM
To: histonet
Subject: Re: [Histonet] RE: RVU units
I would like to see the answer posted on the histonet, too.
Patti Loykasek
PhenoPath Laboratories
Seattle, WA
> I need to set up two tests and need to assign RVU units to two
> CPT codes in order to get the tests I need in our billing system. I
> understand this may be a touchy subject but if anyone is willing to
> share how many RVU units they assigned to the CPT codes for 88189 Flow
> cytometry interpretation and 88358 DNA ploidy Digital image analysis I
> would appreciate it greatly.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> histonet-request@lists.utsouthwestern.edu
> Sent: Wednesday, May 11, 2005 11:10 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 18, Issue 14
>
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> Today's Topics:
>
> 1. Mast cell stain (Osborn, Sharon)
> 2. Slide labelers (Osborn, Sharon)
> 3. Re: Asbestos studies (Gayle Callis)
> 4. Control for Mast cells (Gayle Callis)
> 5. Picrosirius red stain (Jeffrey Wu)
> 6. RE: Control for Mast cells (Jim Staruk)
> 7. Re: mast cell in guinea pig (John Kiernan)
> 8. RE: Asbestos studies (Shirley Powell)
> 9. Final notice: Missouri Society for Histotechnology Spring
> Symposium (Johnson, Teri)
> 10. Master's of Histology (Farley, Sunni R)
> 11. job (cynthia haynes)
> 12. Re: Slide Labeller (MajorFocus@aol.com)
> 13. Re: Mast cell stain (Laurie Reilly)
> 14. Re: job (Patti Loykasek)
> 15. Mast Cell/Giemsa clarification (HSRL)
> 16. RE: Master's of Histology (Kemlo Rogerson)
> 17. (no subject) (eswary)
> 18. EDTA (Bernadette Weston)
> 19. macrophages in mouse FFPE
> (wasielewski.reinhard.von@mh-hannover.de)
> 20. Gayle - floating petri dish snap freezing question
> (Kristen Broomall)
> 21. RE: macrophages in mouse FFPE (Flynn, Evelyn)
> 22. New rabbit antibody... but what do I use as negative
> (isotype) control? (- -)
> 23. RE: job (Margaryan, Naira)
> 24. Unsubscribe (Flores, Teresa)
> 25. Unsubscribe (Flores, Teresa)
> 26. Help!!! (vsailes@nd.edu)
> 27. job listing (LINDA MARGRAF)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 10 May 2005 13:14:25 -0400
> From: "Osborn, Sharon"
> Subject: [Histonet] Mast cell stain
> To: "'histonet@lists.utsouthwestern.edu'"
>
> Message-ID:
> <29B25753F6B1D51196110002A589D44402397F8E@PALMSG30.us.schp.com>
> Content-Type: text/plain
>
> Tom,
>
> Luna's Toluidine Blue Method for Mast Cells is a superior
demonstration
> of
> mast cells using a pink background. These literally pop up under low
> power.
> The method is located on pages 311-312 of Luna's Histopathologic
Methods
> and
> color Atlas of special Stains and Tissue Artifacts; 1992. If you wish
a
> copy of the procedure, private email me and I will send to you.
>
> sharon osborn
> DNAX ScheringPlough BioPharma
> Palo Alto, CA
>
> Subject: [Histonet] Giemsa Stain for Mast Cells
> Netters,
> I am looking for a Giemsa stain for Mast Cells that does not have a
> pinkish
> hue like the Gaffney Giemsa. We are looking to have the Mast Cells
pop
> out
> at us on low power. Any suggestions/protocols?
> Thanks,
> Tom
> Tom Galati
> Laboratory Director
> HSRL, Inc.- A GLP Compliant Contract Laboratory
> 137 South Main Street
> Woodstock, Virginia 22664
> (540)459-8211
> Fax: (540)459-8217
> tomgalati@hsrl.org
> www.hsrl.org
>
>
>
> *********************************************************************
> This message and any attachments are solely for the intended
recipient.
> If you are not the intended recipient, disclosure, copying, use or
> distribution of the information included in this message is prohibited
> -- Please immediately and permanently delete.
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 10 May 2005 13:21:48 -0400
> From: "Osborn, Sharon"
> Subject: [Histonet] Slide labelers
> To: "'histonet@lists.utsouthwestern.edu'"
>
> Message-ID:
> <29B25753F6B1D51196110002A589D44402397F8F@PALMSG30.us.schp.com>
> Content-Type: text/plain
>
> Cynthia,
> Contact your Leica representative and ask for a demonstration of
> their slide printers. I think you will be pleasantly surprised as to
> the
> amount of information and quietness. I have used several different
> slide
> labelers including the etch-a-sketch types and ink printer types; this
> one
> is the best of all I have used. Its footprint is a little larger than
> the
> others and well worth it. Sakura and Leica partnered on developing
> these;
> their main difference is in the software that is provided. Sakura uses
> off
> the shelf software while Leica specifically built the software on
theirs
> and
> it is customizable for stand alone or for integration with MIS of an
> institution. And, the company service will also make a difference.
> So, I encourage you to demo several different types of slide
> labelers and find the one that best suits your needs.
> Sharon Osborn
> DNAX
> ScheringPlough BioPharma
> Palo Alto, CA
>
>
> Date: Tue, 10 May 2005 10:37:23 -0400
> From: "Favara, Cynthia (NIH/NIAID)"
> Subject: [Histonet] slide labeller
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>
> Content-Type: text/plain
>
> I am interested in an automated slide labeler. I would like the
capacity
> to
> do multiple slides with the same information as quiet as possible and
of
> course I have limited space. Any suggestions would be appreciated.
>
>
>
> I also have tops to the bottle for a Ventana special stainer. I am
happy
> to
> send to anyone interested.
>
>
>
> c
>
>
>
> Cynthia Favara
> NIAID/NIH/RML/LPVD
> 903 South 4th Street
> Hamilton, MT 59840
> 406-363-9317
>
>
>
> *********************************************************************
> This message and any attachments are solely for the intended
recipient.
> If you are not the intended recipient, disclosure, copying, use or
> distribution of the information included in this message is prohibited
> -- Please immediately and permanently delete.
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 10 May 2005 11:25:43 -0600
> From: Gayle Callis
> Subject: Re: [Histonet] Asbestos studies
> To: mtitford@aol.com, Histonet@lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20050510112352.01b41688@gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> If I remember correctly, an iron stain will identify asbestos fibers
> nicely
> in a section. Histonet had discussion on asbestos fibers some time
ago,
>
> may be worth a search in archives at www.histosearch.org
>
> At 09:52 AM 5/10/2005, you wrote:
>> Fred Underwood asks about asbestos fibers in lung tissues.
>>
>> Asbestosis and mesothelioma studies are quite big business along the
> Gulf
>> Coast here with the local shipyards and men of the "Golden
Generation"
>> passing away. A lot of lawyers have made a lot of money and most
> recently,
>> a big lawsuit in Corpus Christi Texas accuses screening companies of
>> misdiagnosis.
>> However, onto things histological: asbestos fibers can be easily seen
> in
>> an H&E section as brown, cylindrical, and beaded. In our laboratory
we
>> digest lung tissue in bleach and then filter the remains through a
>> millipore. Once dried, it can be placed on a slide, the filter
> dissolved
>> in chloroform and mounted with a coverslip. The fibers are then
> counted.
>> The reference is Roggli VL, Oury, TD and Sporn TA " Pathology of
>> asbestos-associated diseases" 2ed edition Spinger. 2004
>>
>> Mike Titford
>> USA Pathology
>> Mobile AL USA
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 10 May 2005 11:28:28 -0600
> From: Gayle Callis
> Subject: [Histonet] Control for Mast cells
> To: "Anna Inman" ,
> Histonet@lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20050510112558.01b62930@gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Skin contains mast cells in connective tissues and have seen them in
> connective tissues surrounding bones.
>
> At 10:15 AM 5/10/2005, you wrote:
>> -->
>>
>> I am having difficulty getting a control for Mast cells. Any
> suggestions?
>>
>>
>>
>> Anna Inman B.S., HT (ASCP)
>>
>> SMH Pathology
>>
>> Anna.Inman@stmarygj.org
>>
>>
>>
>>
>>
>>
>>
>> -----Original Message-----
>> From: histonet-bounces@lists.utsouthwestern.edu
>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle
> Callis
>> Sent: Tuesday, May 10, 2005 8:51 AM
>> To: histosci@shentel.net; Histonet@lists.utsouthwestern.edu
>> Subject: Re: [Histonet] Giemsa Stain for Mast Cells
>>
>>
>>
>> Carsons pH 4.3 toluidine blue stain is excellent for Mast cells as is
>>
>> Churukian Shenk method for mast cells. You don't have to worry about
> so
>>
>> many other colors from Giemsa. If you want these, I will be happy to
>>
>> attach privately.
>>
>>
>>
>> At 07:45 AM 5/10/2005, you wrote:
>>
>>> Netters,
>>
>>>
>>
>>>
>>
>>>
>>
>>> I am looking for a Giemsa stain for Mast Cells that does not have a
>>
>>> pinkish hue like the Gaffney Giemsa. We are looking to have the
Mast
>>
>>> Cells pop out at us on low power. Any suggestions/protocols?
>>
>>>
>>
>>>
>>
>>>
>>
>>> Thanks,
>>
>>>
>>
>>>
>>
>>>
>>
>>> Tom
>>
>>>
>>
>>>
>>
>>>
>>
>>> Tom Galati
>>
>>>
>>
>>> Laboratory Director
>>
>>>
>>
>>> HSRL, Inc.- A GLP Compliant Contract Laboratory
>>
>>>
>>
>>> 137 South Main Street
>>
>>>
>>
>>> Woodstock, Virginia 22664
>>
>>>
>>
>>> (540)459-8211
>>
>>>
>>
>>> Fax: (540)459-8217
>>
>>>
>>
>>> tomgalati@hsrl.org
>>
>>>
>>
>>> www.hsrl.org
>>
>>>
>>
>>>
>>
>>>
>>
>>> _______________________________________________
>>
>>> Histonet mailing list
>>
>>> Histonet@lists.utsouthwestern.edu
>>
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> Gayle Callis
>>
>> MT,HT,HTL(ASCP)
>>
>> Research Histopathology Supervisor
>>
>> Veterinary Molecular Biology
>>
>> Montana State University - Bozeman
>>
>> PO Box 173610
>>
>> Bozeman MT 59717-3610
>>
>> 406 994-6367 (lab with voice mail)
>>
>> 406 994-4303 (FAX)
>>
>>
>>
>>
>>
>>
>>
>> _______________________________________________
>>
>> Histonet mailing list
>>
>> Histonet@lists.utsouthwestern.edu
>>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> This electronic message and all contents contain information from St.
>> Mary's Hospital which may be attorney-client privileged,
confidential
> or
>> otherwise protected from disclosure. The information is intended to
be
>
>> for the addressee only. If you are not the addressee, any
disclosure,
>> copy, distribution or use of the contents of this message is
>> prohibited. If you have received this electronic message in error,
> please
>> notify the sender immediately and destroy the original message and
all
>> copies. Thank you
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 10 May 2005 13:30:54 -0400
> From: "Jeffrey Wu"
> Subject: [Histonet] Picrosirius red stain
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; format="flowed"
>
>
> Hello all,
>
> I am trying to quantitate the amount of fibrosis in mouse
> cardiac
> tissue. I based my method on Dr Kiernan's excellent tutorial
> on
> picrosirius red staining (posted in 2000). Briefly, slides
> are
> deparaffinated in xylene (2x, 6 min); 100% ethanol (2x, 3 min);
> 75%
> ethanol (1x, 3 min); and running distilled water (10-15 min).
> Next,
> they are stained in saturated picrosirius red for 60 minutes
> and
> washed in acetic acid (2x, 3 min). They are dehydrated in 75%
> ethanol
> (1x, 3 min); 100% ethanol (2x, 3 min); and xylene (1x, 3
> min).
> Finally, the slides are mounted with Permount.
>
> For image processing, I am using circularly polarized light.
> Under
> the microscope, the collagen stains from pink to dark red
> (almost
> black), depending on its content; however, the background
> tissue
> appears green/blue. It is problematic because I quantify
> the
> collagen using ImagePro Plus software, in which I select
> certain
> colors. Especially with the pink and some dark blues, there
> is
> overlap between the collagen and tissue, causing overestimation
> of
> collagen. (Sorry for the long explanation.) I guess what I
> am
> seeking is the flaw in my procedure, causing the other tissue not
> to
> stain yellow.
>
> On a side note, I am using the correct picrosirius red stain, and
> the
> solution is saturated with crystals at the bottom. I
> have
> tried modifying washing times without any change. I used a short
> (2
> min) 0.2% phosphomolybdic acid wash, which is supposed to
> reduce
> background, with no success as well.
>
> Once again, sorry for the long question. Any help with my
> problem
> would be greatly appreciated. Please do not hesitate to email me
> with
> any questions or anything that I have not made clear. Thank you
> in
> advance.
>
> J Wu
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 10 May 2005 13:52:27 -0400
> From: "Jim Staruk"
> Subject: RE: [Histonet] Control for Mast cells
> To: "'Anna Inman'" ,
>
> Message-ID: <0IGA002BACE3E8G4@vms048.mailsrvcs.net>
> Content-Type: text/plain; charset=us-ascii
>
> Anyone in diagnostic veterinary services gets mast cell tumors all of
> the
> time. I'll be glad to share some with someone in need (and I won't
even
> charge you).
>
> Jim
>
> ______________________
> Jim Staruk
> Mass Histology Service
> www.masshistology.com
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle
> Callis
> Sent: Tuesday, May 10, 2005 1:28 PM
> To: Anna Inman; Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Control for Mast cells
>
> Skin contains mast cells in connective tissues and have seen them in
> connective tissues surrounding bones.
>
> At 10:15 AM 5/10/2005, you wrote:
>> -->
>>
>> I am having difficulty getting a control for Mast cells. Any
> suggestions?
>>
>>
>>
>> Anna Inman B.S., HT (ASCP)
>>
>> SMH Pathology
>>
>> Anna.Inman@stmarygj.org
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 10 May 2005 13:58:36 -0400
> From: John Kiernan
> Subject: Re: [Histonet] mast cell in guinea pig
> To: Histonet
> Message-ID: <4280F64C.CD1F5085@uwo.ca>
> Content-Type: text/plain; charset=us-ascii
>
>
>
> John Kiernan wrote:
>>
>> Probably you didn't see any mast cells in the
>> sections of guinea-pig tissues because there
>> were none to be seen.
>>
>> Mouse and rat mast cells are unusual in that their
>> granules are preserved and rendered insoluble by
>> aqueous fixatives such as buffered formaldehyde.
>> In most other species aqueous fixatives dissolve
>> out the mast cell granules. Alcoholic fixatives (eg
>> Carnoy, or the "alcoholic Bouin" fluids: Dubosq, Gendre
>> etc) will preserve and insolubilize mast cell granules
>> of any species.
>>
>> For more on this, see "The Mast Cells" by Hans Selye
>> (1965) Chapter 3. Also the "Notes & Queries section in
>> the current issue of Biotechnic & Histochemistry (Vol 80
>> No 1, p.43-45). The latter is available at the publisher's
>> web site:
>>
>
http://journalsonline.tandf.co.uk/app/home/journal.asp?wasp=5a0cd1389c12
>
4b68b94584a9e888d3f3&referrer=parent&backto=searchpublicationsresults,1,
> 1;homemain,1,1;
>>
>> The metachromasia is due to heparin, the major
>> macromolecular anion of the granules. The staining
>> properties of heparin are similar to those of
>> cartilage matrix. Both materials carry a lot
>> of sulphate-ester groups. Unfortunately heparin
>> (except that of rats and mice) is water-soluble.
>> I do not know if any tryptase or chymase immunoreactivity
>> remains in mast cells whose granules have been
>> extracted by an aqueous fixative.
>> --
>> -------------------------------
>> John A. Kiernan
>> Department of Anatomy and Cell Biology
>> The University of Western Ontario
>> London, Canada N6A 5C1
>> kiernan[AT]uwo.ca
>> http://publish.uwo.ca/~jkiernan/
>> http://instruct.uwo.ca/anatomy/530/index.htm
>> _______________________________
>> Elizabeth Chlipala wrote:
>>>
>>> Hello All
>>>
>>> I'm looking for a stain that will identify mast cells in guinea
> pigs. I
>>> have run both giemsa (modified dif quick) and 0.4% Toluidine Blue.
> I
>>> normally get very good staining of mast cells with the toluidine
> blue.
>>> I normally run this stain on mouse and rat tissue. This is the
> first
>>> time I have tried this stain in guinea pigs. Upon review of the
> slides
>>> I could not located one mast cell in 11 lung sections. I know they
> have
>>> to be there. Does the metachromatic nature of the t. blue stain
> have to
>>> do with the amount of histamine present in the cells? I have read
> in
>>> the literature that guinea pigs and humans have less histamine
> present
>>> in their mast cells than rats, hamsters and mice. Is anyone aware
> of a
>>> modification that will stain mast cells in guinea pig? Any help
> would
>>> be appreciated. I would prefer to stick with a histochemical
> method.
>>> I'm aware of Immunohistochemical staining for mast cell tryptase,
> but in
>>> researching this I could not find any references for guinea pig
> tissue.
>>>
>>> Thanks in advance.
>>>
>>> Liz
>>>
>>> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>>> Manager
>>> Premier Laboratory, LLC
>>> P.O. Box 18592
>>> Boulder, Colorado 80308
>>> Office: (303) 735-5001
>>> Fax: (303) 735-3540
>>> liz@premierlab.com
>>> www.premierlab.com
>>>
>>> Ship to Address:
>>> Premier Laboratory
>>> University of Colorado
>>> MCDB, Room A3B40
>>> Boulder, Colorado 80309
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet@lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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