[Histonet] Vector Red and True Blue
Hello All,
I have been attempting some double labeling for fos and estrogen receptor
for about a month with little luck. I am using Vector Red (fos) and True
Blue (ER) for my staining. I am having no problems with the ER labeling,
but the fos labeling is not that clear. I don't appear to be getting a
strong reaction product from the Vector Red. Has anyone else had this
problem? How strong should the staining be (light pink, dark pink, red)? I
have been careful to only place in xylene for a few moments before
coverslipping and therefore don't think fading is my problem.
I have been thinking that my antigen retrieval may be the problem. I am
using triton X to improve antigen retrieval, but was thinking about
microwaving. These are free floating sections. First is this possible to
do with free floating sections and second how long do I microwave? Any
help for this lost soul is greatly appreciated.
Ben
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