[Histonet] New rabbit antibody... but what do I use as negative (isotype) control?

From:- -

Hi everyone,
 
I have a new antibody that I was given.  It is a rabbit polyclonal anti-mouse antibody.  Usually I use a specific isotype control, but I do not have info on how this antibody was made other than the epitope(s) that it should adhere to...  but I want a negative control and was wondering if I can just use regular rabbit serum (diluted) as a negative seeing that it should contain the isotype IgG I am using??? I think I am a bit confused here.... I need assistance.
 
Can I use rabbit serum as a negative control?
What dilution do I use if that is the case?
Anything else I should know before I proceed?
 
Thank you all!!
 
 

		
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