[Histonet] Background!

From:pex

Hello, all,
 
I am doing double Immunofluorescence in bone sections. But I find there is so much backgound in sections, I have used the normal serum matched to the host of secondary antibodies for blocking, moreover I have also add times for washing, so I do not really know how to reduce the background. Can anybody do me a favor?Thank you!
 
Best wishes!
 
Guofeng


Guofeng


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