Hi There

Can anybody shed some light onto the subject of crystals forming 
after the use of BCIP/NBT on alk phos staied tissue. At first when I 
mount the slides, you do not see the precipitate, btu within hours, 
it deveopes. I must add that the tissue is ZINC fixed. As zinc is a 
precippitant, I am not sure where to look for the problem. DAKO has 
no idea!!


This problem has been discussed before, but I cannot recall if it was 
ever resolved.

Many Thanks
Melanie Black
-- 
Cardiovascular Research Unit
Div. of Cardiothoracic Surgery
Chris Barnard Building
University of Cape Town
Anzio Road
Observatory
7925
Republic of South Africa

Tel +27 21 406-6589
Cel +27 82 469-3352
Fax +27 21 448-5935

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet