Hello, all,
 
alkaline phosphatase ( ALP) is a marker gene of osteoblast, it is mainly expressed in cytoplasm in vitro. Recently I do immunofluorescence in bone sections, but I donot find positive expression in osteoblasts, only it is found in hypertrophic cartilage and part bone marrow, so I am not sure that where ALP is located in bone sections. If you have some experience in it, can you do me a favor?
Thank you!
 
Guofeng



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