Re: [Histonet] Osmium Tetroxide Protocol

From:Lesley Weston

It's better than nothing, but not really adequate. OsO4 is really nasty
stuff; you should treat it with great caution. A mask won't help at all,
unless you mean a full-scale respirator - it's fumes you're worried about,
not particles - but you should certainly wear gloves and a lab-coat, and
change the gloves frequently. If you can't use a real fume hood,
properly-fitting goggles might be an idea, too; the fumes can fix the cornea
permanently, and possibly even affect the retina. Do you have the MSDS sheet
for OsO4? That would give you all the information you need.

Lesley Weston.

on 25/05/2004 12:06 PM, at wrote:

> Hi Histonetters,
> When handling Osmium Tetroxide, is a chemical fume gard such as an Bench Top
> fume absorber appropriate to use for this chemical?
> Also, should you wear other PPE besides gloves, gown etc. Should you wear a
> mask also?
> Thank you.
> Marsha Price
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