Re: [Histonet] IHC_swine vessels_cross reactivity

From:Gayle Callis

You can try to find the antibodies at SEROTEC, they can tell you what cross
reacts to swine from their cross reactivity chart.  This may be on their
website.  If the resin is Glycol methacrylate plastic, you will will have
poor success with immunostaining.  IF the tissue is embedded in
methylmethacrylate, you can use Neil Hands methods for retrieval, and
remove the plastic COMPLETELY.  This has been discussed on Histonet before,
you can do a search in Histonet archives.


At 09:39 AM 5/31/2004 +0530, you wrote:
>Dear all,
>I need to identify macrophages and endothelial cells in resin embedded 
>pig blood vessels with stent. I couldnt find any specific primary 
>antibodies against swine macrophages or endothelial cells and has to 
>rely on cross reactivity pirmarily intended in human tissue. Has any one 
>tried such cross reactive antibodies in swine tissue or is there 
>specific ones against pig which ive missed. Please help
> J. Raghul, Scientist -C, sri chithra tirunal institute of medical 
> sciences, Trivandrum , India
>Histonet mailing list
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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