RE: [Histonet] exotic species/chief&parietal cells
I remembered Harry Cook when you posted your question. He always had very
good transparencies when he gave his lectures and I thought he showed us
sections of just what you are looking for so I had a look through his
book:Histological Demonstration Techniques. Here are the methods he
PAS-Toluidine Blue-Aurantia (Cook, 1962, Stain Technology 37, 317) and
Methasol Fast Blue-PAS-Alcian Yellow (Maxwell, 1963, Stain Technology, 38,
286). I haven't tried these, but if Cook recommended them they should be OK.
I attach the methods, but I don't know if you can still get all the
Good luck anyway.
University of Cambridge
[mailto:email@example.com]On Behalf Of Marshall
Sent: Wednesday, May 26, 2004 11:51 AM
Subject: [Histonet] exotic species/chief&parietal cells
We are looking at the gastric mucosa of the Cape Mole Rat and can not
distinguish chief cells on the basis of morphology. There seem to be
more parietal cells and we can not be sure of the chief cells. Is there
a stain we can use to distinguish between these to cell types as the
Hematoxylin & Eosin stain is not making this obvious.
Dept. Human Biology
University of Cape Town
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