RE: [Histonet] caspase 3 and pcna

From:"Elizabeth Chlipala"

I have done caspase 3 staining on some organotypic cultures  (EpiOralT),
but these have been processed and embedded into paraffin.  The antibody
I use is from cell signaling technologies, catalog #9661.  I have also
used this antibody on rat and mouse specimens.
Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
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University of Colorado
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Boulder, Colorado 80309
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-----Original Message-----
[] On Behalf Of Patsy
Sent: Thursday, May 27, 2004 12:09 PM
To: Jackie.O'
Subject: [Histonet] caspase 3 and pcna
Jackie and all, could you please advise me on doing caspase 3 and pcna
cells grown on chamber slides, I mostly need advise with caspase, what
antibody do you use?
Thank you all,
-----Original Message-----
[]On Behalf Of
Sent: Friday, May 21, 2004 8:11 AM
Subject: Re: [Histonet] Embedding
I worked in a derm lab for a few years, and it seemed best to embed the
skins on a slight angle to the mold, so that when you cut through the
tissue first, your knife wasn't hitting a straight line of (sometimes)
hard epidermis.  Also, if you cut through the epidermis first, there is
tendency to damage the knife edge, or drag hard particles through the
tissue,  creating scratch artifacts in your section.
Jackie O'
Sent by:
05/21/2004 08:46 AM
        Subject:        [Histonet] Embedding
I have a question for all of the derm and hard tissue people out there.
do you embed the sections?  Do you cut the dense or hard tissue first or
- or do you embed at an angle?  One individual is questioning the answer
in the study guide and so far I have different answers from those I have
Freida Carson
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