RE: [Histonet] caspase 3 and pcna

From:"Elizabeth Chlipala"

Patsy
 
I have done caspase 3 staining on some organotypic cultures  (EpiOralT),
but these have been processed and embedded into paraffin.  The antibody
I use is from cell signaling technologies, catalog #9661.  I have also
used this antibody on rat and mouse specimens.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala@premierhistology.com
www.premierhistology.com
 
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
 
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Thursday, May 27, 2004 12:09 PM
To: Jackie.O'Connor@abbott.com
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] caspase 3 and pcna
 
Jackie and all, could you please advise me on doing caspase 3 and pcna
on
cells grown on chamber slides, I mostly need advise with caspase, what
antibody do you use?
Thank you all,
Patsy
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Jackie.O'Connor@abbott.com
Sent: Friday, May 21, 2004 8:11 AM
To: FreidaC@aol.com
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Embedding
 
 
I worked in a derm lab for a few years, and it seemed best to embed the
skins on a slight angle to the mold, so that when you cut through the
soft
tissue first, your knife wasn't hitting a straight line of (sometimes)
hard epidermis.  Also, if you cut through the epidermis first, there is
a
tendency to damage the knife edge, or drag hard particles through the
soft
tissue,  creating scratch artifacts in your section.
Jackie O'
 
 
 
 
FreidaC@aol.com
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/21/2004 08:46 AM
 
 
        To:     histonet@lists.utsouthwestern.edu
        cc:
        Subject:        [Histonet] Embedding
 
 
I have a question for all of the derm and hard tissue people out there.
How
do you embed the sections?  Do you cut the dense or hard tissue first or
last
- or do you embed at an angle?  One individual is questioning the answer
given
in the study guide and so far I have different answers from those I have
consulted.
 
Thanks,
 
Freida Carson
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