RE: [Histonet] anti-CD11b

From:"Patsy Ruegg"

Liz is right about BD CD11b, I never was able to get it to work on ffpe
mouse tissue, I have although had some limited success with DAKO CD68 PGM1
clone which only stains macrophages and monocytes and not all the other
myleoid cells, but it is not easy and inconsistant in my hands.

-----Original Message-----
[]On Behalf Of Elizabeth
Sent: Thursday, May 27, 2004 10:02 AM
Subject: RE: [Histonet] anti-CD11b


We have used BD's rat anti mouse CD11b.  (Cat Number: 550282).  This
antibody will only work on frozen sections and unless things have
changed over the period of a year I'm not aware of any CD11b marker for
mouse that will work on FFPE tissues.  If there is anyone out there that
knows different please reply.  I have a protocol and images if you like.


Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540

Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309

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-----Original Message-----
[] On Behalf Of Baowei
Sent: Thursday, May 27, 2004 3:28 AM
Subject: [Histonet] anti-CD11b

Dear all,

Would anyone recommend a rat anti mouse CD11b mAb to to identify
monocytes, possibly can be used both in IHC and FC.
Thanks in advance!

Baowei Peng

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