How are your fresh cells fixed?  In my experience aldehyde fixed paraffin
processed tissue especially decalcified is not the best for demonstrating
osteoclasts with TRAP, although Jackie O'Connor at Abbott has gotten it to
work on formalin fixed tissue if the fixation was not very long.  I use TRAP
on cold methanol fixed bone biopsie's processed without decalcification into
glycol methacrylate.  I would think that the fresh fixed cells should work
fine.
Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Myri37@aol.com
Sent: Tuesday, June 01, 2004 8:11 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] TRAP staining


Hi
I would like to try SIGMA kit N°85, for staining osteoclats on fresh fixed
cells (reconstructed tissue, and also paraffin embedded secions. Tissue is
fixed in 4 % paraformaldehyde, is there another fixative wich works better ?
Do you think if i must change the way to proceede ?

Thank you very much
Myriam baali
Natural implant


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