RE: [Histonet] Re: chicken in situ hybridization
Thank you for your points. In my protocol, I treat the slides 5min
with proteinase K, but the my chicken embryo is E4. Maybe I should
shorten the time. Also, I want to ask how you do the hybridization
step. In our lab, we drop 100ul hybridization solution with probe on
the slide and then put a coverslip on it. The day after, we remove the
coverslip by dipping the slides in the ssc solution. I find it is also
a reason for losing the structure. Do you have other way to do it?
>From: "Johnson, Teri"
>Subject: [Histonet] Re: chicken in situ hybridization
>Date: Mon, 24 May 2004 08:40:16 -0500
>Are you using proteinase K enzyme digestion in your protocol? You
>not need it on very young chick embryo cryosections. We've found
>we don't need it most times on E2 whole mount ISH.
>Managing Director Histology Facility
>Stowers Institute for Medical Research
>1000 E. 50th St.
>Kansas City, Missouri 64110
>Date: Sun, 23 May 2004 15:17:41 +0000
>From: "dengqiaolin deng"
>Subject: [Histonet] chicken in situ hybridization
> Dear histonetter:
> Is there anyone having done in situ hybridization for
> cryosection? One kind of problems I have met is that the
> easy to be crashed and disattached from the slide although I am
> careful with every washing steps. But for the mouse
> everything is fine. Is there any improvement that I can do to
> Thank you for any suugestions in advance.
> Qiaolin Deng
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