One of my former students, James Gentile (now a prosthodontist in
Philadelphia), got excellent results by using a fine gauge hypodermic needle
to withdraw some of the vitreous humor and injecting Bouin's fluid into the
vitreous chamber.  He then immersed the eye in Bouin's for a few days.
   You might get similar results by injecting 4% paraformaldehyde into the
vitreous chamber.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gareth Davis
Sent: Tuesday, May 25, 2004 5:10 PM
To: Histonet
Subject: [Histonet] Good cryosections of rat retina?

Hello all,
This week I am trying to get good cryosections of rat eyes.  We are mostly
interested in the retinas.  The eyes I have were from animals perfused with
4% Paraformaldehyde, eyes were post-fixed with same, then cryopreserved.
Eyes were placed in OCT for cutting.  The eyes were not cut in half before
placing in OCT.  I am having a little trouble getting good sections.
I would just like to get some ideals of how others prepare animal eyes for
sectioning.
Thanks,
Gareth Davis
Research Assistant
Tennessee State University
Nashville, Tennessee
 

		
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