RE: [Histonet] Counterstain to use with blue and redsubstrates

From:Margaret Blount

Sigma Fast Fast Red TR/maphthol AS-MX phosphate also fluoresces red. In case
you are a Sigma fan. 


Margaret Blount
Chief Technician
Clinical Biochemistry
University of Cambridge
Addenbrooke's Hospital
Hills Road
CB2 2QR 

-----Original Message-----
[]On Behalf Of Gayle
Sent: Friday, May 28, 2004 5:04 PM
To: Donna Kumiski;
Subject: Re: [Histonet] Counterstain to use with blue and redsubstrates

One can purchase Vectashield containing DAPI, it is aqueous mounting media
(also comes in a form that sets up hard!), coverslip and stain with DAPI
all at once!  Very tidy.  If you use VECTOR red as a chromogen, it
fluoresces with UV light AND appears red via light microscopy.  To have
crisp Vector Red (I think the new DAKO red chromogen also fluoresces) be
sure you follow brochure directions AND add Tween 20 to buffer when making
up Vector Red. The best of both worlds.  

At 08:40 AM 5/28/2004 -0400, you wrote:
>What about using a fluorescent counter stain such as DAPI? You would of
>course have to
> have the correct filter to view it. I have included a link with the
>details of the stain.
> Donna
>Donna Kumiski     HT (ASCP)
>Medical College of Georgia
>Dept. of Cellular Biology and Anatomy
>CB 1202
>Augusta, Georgia   30912-2000   
>(706) 721-6278
>Histonet mailing list
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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