Jackie and all, could you please advise me on doing caspase 3 and pcna on
cells grown on chamber slides, I mostly need advise with caspase, what
antibody do you use?
Thank you all,
Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Jackie.O'Connor@abbott.com
Sent: Friday, May 21, 2004 8:11 AM
To: FreidaC@aol.com
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Embedding


I worked in a derm lab for a few years, and it seemed best to embed the
skins on a slight angle to the mold, so that when you cut through the soft
tissue first, your knife wasn't hitting a straight line of (sometimes)
hard epidermis.  Also, if you cut through the epidermis first, there is a
tendency to damage the knife edge, or drag hard particles through the soft
tissue,  creating scratch artifacts in your section.
Jackie O'




FreidaC@aol.com
Sent by: histonet-bounces@lists.utsouthwestern.edu
05/21/2004 08:46 AM


        To:     histonet@lists.utsouthwestern.edu
        cc:
        Subject:        [Histonet] Embedding


I have a question for all of the derm and hard tissue people out there.
How
do you embed the sections?  Do you cut the dense or hard tissue first or
last
- or do you embed at an angle?  One individual is questioning the answer
given
in the study guide and so far I have different answers from those I have
consulted.

Thanks,

Freida Carson
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