[Histonet] Re: chicken in situ hybridization
Are you using proteinase K enzyme digestion in your protocol? You may
not need it on very young chick embryo cryosections. We've found that
we don't need it most times on E2 whole mount ISH.
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
Date: Sun, 23 May 2004 15:17:41 +0000
From: "dengqiaolin deng"
Subject: [Histonet] chicken in situ hybridization
Is there anyone having done in situ hybridization for
cryosection? One kind of problems I have met is that the structure
easy to be crashed and disattached from the slide although I am
careful with every washing steps. But for the mouse
everything is fine. Is there any improvement that I can do to
Thank you for any suugestions in advance.
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