[Histonet] Good cryosections of rat retina?
Hello all,
This week I am trying to get good cryosections of rat eyes. We are mostly interested in the retinas. The eyes I have were from animals perfused with 4% Paraformaldehyde, eyes were post-fixed with same, then cryopreserved. Eyes were placed in OCT for cutting. The eyes were not cut in half before placing in OCT. I am having a little trouble getting good sections.
I would just like to get some ideals of how others prepare animal eyes for sectioning.
Thanks,
Gareth Davis
Research Assistant
Tennessee State University
Nashville, Tennessee
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