[Histonet] BK virus detection.

From:"Bill Sinai"


Dear Histonetters,

I am writing for a colleague who performs all our Immunohistochemistry.
She is asking:
"Can anyone help us with a reliable method of the detection of BK virus in
Renal Transplant biopsies.
We use a Chemicon anti-BK virus and treat the sections in citrate buffer in
a pressure cooker in the microwave. A polymer based peroxidase kit is used
for detection.  Unfortunately this method gives us a great deal of
non-specific staining and almost every nucleus of every cell in the tissue
is stained, no what the dilution of the antibody.
We would be grateful if anyone can impart the secret of their success with
us.

Many thanks
Jane"

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


__________________________________________________________________

This electronic message and any attachments may be confidential.  If you
are not the intended recipient of this message would you please delete the
message and any attachments and advise the sender. Western Sydney
Area Health Services (WSAHS) uses virus scanning software but excludes 
any liability for viruses contained in any email or attachment.

This email may contain privileged and confidential information intended
only for the use of the addressees named above. If you are not the
intended recipient of this email, you are hereby notified that any use,
dissemination, distribution, or reproduction of this email is prohibited. If
you have received this email in error, please notify WSAHS
immediately. 

Any views expressed in this email are those of the individual sender 
except where the sender expressly and with authority states them 
to be the views of WSAHS.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>