[Histonet] BK virus detection.

From:"Bill Sinai"

Dear Histonetters,

I am writing for a colleague who performs all our Immunohistochemistry.
She is asking:
"Can anyone help us with a reliable method of the detection of BK virus in
Renal Transplant biopsies.
We use a Chemicon anti-BK virus and treat the sections in citrate buffer in
a pressure cooker in the microwave. A polymer based peroxidase kit is used
for detection.  Unfortunately this method gives us a great deal of
non-specific staining and almost every nucleus of every cell in the tissue
is stained, no what the dilution of the antibody.
We would be grateful if anyone can impart the secret of their success with

Many thanks

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Ph 02 9845 7774


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