[Histonet] BCL6 w/o EDTA
If this helps, we use DAKO Hi pH retrieval solution in a steamer for 20 min. We use Envision for a detection kit as the Hi pH retrieval solution yeilds a lot of biotin staining. We use Novocastra primary ab at 1:5. Our results are good and if you use a really good charged slide and dry the sections well before staining the morphology is fine.
Date: Fri, 21 May 2004 13:46:27 -0400
From: Browning Deb
Subject: [Histonet] BCL6, EDTA buffer
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Good afternoon: As much as I dislike EDTA buffer due to the poor morphology
(especially if not properly fixed) that it renders, I have not had much
success with BCL6 staining with any other buffers. I would appreciate any
information on how to improve the morphology of the tissue, the use of other
buffers, or any success stories, since I just can't seem to pull the rabbit
out of the hat here. Thank you.
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