Dear Luz:

Luz Cardenas wrote:

>Hi everyone,
>1.Can cytochrome oxidase method stain fixed tissue (4% Paraformaldehyde)?
>
Yes, provided the fixation is short. Very short. Fix fresh frozen=20
sections 5 min in cold buffered formalin. Whole animal perfusion for 15 
seconds followed by a buffered sucrose washout in preparation for frozen 
sections.

>2. Is there any specific method for fixed tissue?
>
Lots of published methods. See John Kiernan's book (which you should=20
have in the lab) or papers by Margaret Wong-Riley. I don't know if the 
"standard" histopath texts would have this method, perhaps too specialized?

Geoff

>
>Thank you very much in advance!
>Luz.
>
>Luz Cárdenas, MD
>Department of Anatomy and Neurobiology
>University of Tennessee Health Science Center
>855 Monroe Avenue, Room 309/302
>Memphis, TN 38163
>Lab: (901) 448-6002 / 7318
>Fax: (901) 448-7193 (Dept)
>lcardenas@utmem.edu
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>  
>

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
**********************************************





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet