Re: [Histonet] cutting rat brains
From: | Stephen.Eyres@sanofi-synthelabo.com |
Hi David,
Creases come from waterbath temp being too low. Tissue separation can be
caused be water temp being too high and/or you have left the block on water
too long.
Cheers
Steve
"David Clark"
To: Histonet@lists.utsouthwestern.edu
Sent by: cc:
histonet-bounces@lists.utsouth Subject: [Histonet] cutting rat brains
western.edu
03/05/2004 14:39
Hello,
I was wandering if any of you wonderful Histonet people who have much
experience cutting rat brains can offer some assistance in trying to cut
good quality sections. We either encounter lots of wrinkles or tissue
seperation from the paraffin sections once placed on the waterbath. Is
this a processing problem or do you think it is strictly a sectioning
problem?
Any suggestions would be appreciated.
Thanks,
David Clark
Neuropathology
OHSU
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