Re: [Histonet] cryoprotection before MMA embedding at =?iso-8859-1?Q?=2D20=B0C?=

From:John Kiernan

If the -20C is for the methyl methacrylate infiltration
you won't need cryoprotection because by that stage there
shouldn't be any water in the specimens. You have to go
down below -114C to freeze absolute alcohol or -94C to
freeze acetone.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
_______________________________ wrote:
> I found this protocol in reference of a publication of Erben RG, 1997
> it's about "embedding of bone in methylmetacrylate an improved method
> suitable for bone in histomorphometry, histochemitry, and immunohistochemitry"
> embedding is done at -20C that is why i think it needs a cryoprotection.
> it's the first time a lost all tissue, and i dont understand why.
> thank you
> Myriam
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