If the -20C is for the methyl methacrylate infiltration
you won't need cryoprotection because by that stage there
shouldn't be any water in the specimens. You have to go
down below -114C to freeze absolute alcohol or -94C to
freeze acetone.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Myri37@aol.com wrote:
> 
> I found this protocol in reference of a publication of Erben RG, 1997
> it's about "embedding of bone in methylmetacrylate an improved method
> suitable for bone in histomorphometry, histochemitry, and immunohistochemitry"
> embedding is done at -20°C that is why i think it needs a cryoprotection.
> it's the first time a lost all tissue, and i dont understand why.
> thank you
> Myriam
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