Re: [Histonet] Ventana's Confirm CD10?

From:"Joanne Mauger"

Hi Renee!
We are your old budies from HUP, now at CHOP! We use CD10 monoclonal from Santa Cruz. I know you're stuck with Ventana, so try a higher pH buffer for retreival. We use Dako's pH 9.5 for CD10 at 1:25. A CLL tonsil or LN is a good control. Rita and Carol, and me, Joanne, say hi and how are you? How are the twins?
Good luck,
Jo Mauger

>>> "david moses"  05/11/04 02:33PM >>>
I am working up Ventana's Confirm CD10 antibody.The
germinal centers of the normal tonsil are very pale, 
the follicular lymphoma is a little better and the kidney
staining is good. I'm using Trilogy(EDTA) in a Decloaker
for 4 min. at 120c, then a 10 min. cool down.  Any suggestions on how I can improve the staining in my
lymphoid tissue? Thanks.
                                           Renee Boston-Moses HT

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