Re: [Histonet] Luxol Fast blue
Luxol fast blue works well on frozen sections.
You may need a longer differentiation in 0.05%
lithium carbonate than with paraffin sections.
Neutral red provides a contrasting Nissl stain
and also darkens the myelin already coloured by
the luxol fast blue.
Michael Kurnellas wrote:
> I was told that Luxol fast blue staining should be done on paraffin embedded
> tissue. Can I also do this stain on sucrose-embedded tissue, frozen, and
> then placed in OCT compound before 10um sections were taken?
> Mike K.
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