Luxol fast blue works well on frozen sections.
You may need a longer differentiation in 0.05%
lithium carbonate than with paraffin sections.
Neutral red provides a contrasting Nissl stain
and also darkens the myelin already coloured by
the luxol fast blue. 
                       John Kiernan
                       London, Canada
_____________________________________
Michael Kurnellas wrote:
> 
> I was told that Luxol fast blue staining should be done on paraffin embedded
> tissue.  Can I also do this stain on sucrose-embedded tissue, frozen, and
> then placed in OCT compound before 10um sections were taken?
> 
> Thanks.
> Mike K.
> 
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