Re: [Histonet] H&E stain problems
Hi,
I use Harris's hematoxylin which is also regressive and purchase my
hematoxylin and eosin /alcohol-based/ from www.surgipath.com.
I get very good H&E staining with this protocol.
REAGENT TIME
Xylene 3 min.
Xylene 3 min.
Abs. alc. 2 min.
Abs. alc. 2 min
95% alc. 2 min
80% alc. 2 min
Wash /tap water/ 30 sec.
Hematoxylin 8 min.
Wash /tap water/ 2 min.
Acid alcohol 6 sec.
Wash /tap water/ 2 min.
Wash /tap water/ 5 min
80% alc. 30 sec.
Eosin 15 sec.
95% alc. 10 sec.
Abs. alc. 30 sec.
Abs. alc. 30 sec.
Xylene 1 min.
Xylene 1 min.
Xylene Exit
Hope it helps!
Petia
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