Re: [Histonet] Alternative fixative users (Prefer)


Thanks Pattie,
   I have seen first hand what happens with alcohol and ISH and Fish. IHC isn't the problem but when ISH or Fish was orded to back up ISH results. The outcome was a diaster. Thanks for bringing this out.
Tere Hodges 
St. Mary's Hospital
Tucson, Az
  ----- Original Message ----- 
  From: Patti Loykasek 
  To: histonet 
  Sent: Wednesday, May 19, 2004 11:35 AM
  Subject: Re: [Histonet] Alternative fixative users (Prefer)

  I would caution any using Prefer to consider if they will be doing any
  rna/dna testing now or perhaps in the future on the tissue. We have had poor
  dna FISH results with it. Soon I'll be trying some pcr assays, and will let
  you know how that goes.

  Patti Loykasek
  PhenoPath Laboratories
  Seattle, WA

  > Hi,  after many years as a Prefer fixative user our pathologists are telling
  > us that we have to go back to formalin.  This came about after a letter to the
  > editor appeared in the Journal of Surgical Pathology.
  > Has anyone out there been approached by their pathologists to switch back?
  > Mary Bryhan
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