Dawn.

For small or thin biopsies, you probably don't need the vacuum. You are
probably over processing the tissues.

Try shortening the time/dropping the temp a few degrees. The vacuum
attachment is brilliant for thicker blocks.

Could you post some pics to the Histonet Pics site so we could have a look?

	"We can now post images at our web site
(http://pathcuri1.swmed.edu). To have an
image posted send it to Herb Hagler at herb.hagler@email.swmed.edu."

Hope this helps,

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html



-----Original Message-----
From: Dawn Cowie [mailto:dlcowie@prodigy.net] 
Sent: Thursday, 13 May 2004 4:42 AM
To: histonet
Subject: [Histonet] microwave processing


hi histonetters,
Is anyone using the RHS-1 microwave tissue processor with vacuum? If so,
would you care to share with me the processing schedule you use for tiny
bx's such as gastric, liver cores, etc.
We have not been having great success so far. Any help would be appreciated.
Thanks,
Dawn Cowie  HT
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