RE: [Histonet] lymph nodes

From:"Bill Sinai"

In our laboratory, as in many Australian laboratories, we use Feather
Disposable Trimming Knives (Cat #130 in a blade holder Cat# FS130S).  These
are very sharp and easy to use.  Specimen dissection is performed using
these blades for ALL specimens.  They prove to be very beneficial as they
allow those performing specimen dissection to cut reasonably thin slices
(although sometimes to large in other dimensions) from all the specimens we
receive.  Even when performing Frozen Sections on fresh lymph nodes (our
normal triage procedure) we find the registrar is able to cut reasonably
thin blocks of tissue, which then freeze rapidly and evenly.
Being so far away from everywhere else in the world we find that cost is a
factor but these knives prove to be cost effective as they last longer than
scalpels and hand sharpening is time consuming.

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Ph 02 9845 7774

-----Original Message-----
[]On Behalf Of Hawkins,
Hal K.
Sent: Friday, 07 May 2004 7:57 AM
To: Smith, Allen; Marshall Terry Dr, Consultant Histopathologist
Subject: RE: [Histonet] lymph nodes

My preference (mainly for cutting tough samples of skin) is the Weck
prep razor blade -- very sharp, with a well-protected opposite edge.

Hal Hawkins, UTMB Galveston

-----Original Message-----
[] On Behalf Of Smith,
Sent: Thursday, May 06, 2004 11:39 AM
To: Marshall Terry Dr, Consultant Histopathologist
Subject: RE: [Histonet] lymph nodes

Most drugstores still carry old fashioned single edge razor blades which
have a metal strip folded over the dull edge.  They are much easier to
dissect with than the disposable microtome blades. I use them for block

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida

-----Original Message-----
[] On Behalf Of Marshall
Terry Dr,Consultant Histopathologist
Sent: Wednesday, May 05, 2004 11:37 AM
To: Mike Bromley; Histonet (E-mail)
Subject: RE: [Histonet] lymph nodes

I find for this sort of thing, disposable microtome blades are ideal.
Holders are available, but I neither use nor recommend them.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire

-----Original Message-----
From: Mike Bromley []
Sent: 05 May 2004 16:26
To: Histonet (E-mail)
Subject: [Histonet] lymph nodes

Hi Everyone

I am after a very thin blade to slice unfixed lymph nodes, does anyone
any ideas?

Ideally some kind of holder for old style razor blades would be the

Best Wishes

Mike Bromley

Chief Biomedical Scientist
Dumfries & Galloway Royal Infirmary
Scotland, UK

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