RE: [Histonet] cutting rat pancreas
You mention a glycerin solution, what concentration of glycerin? All these
tips are very helpful.
Thanks
Margaret
Margaret Blount
Chief Technician
Clinical Biochemistry
University of Cambridge
Addenbrooke's Hospital
Hills Road
Cambridge
CB2 2QR
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
KAELPERS@aol.com
Sent: Friday, May 07, 2004 4:24 AM
To: pstefanova@sten.sunnybrook.utoronto.ca;
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cutting rat pancreas
Pancreas tissue is always a problem...we have tried cutting larger sections,
blocking them with other tissue, and they always seem to be a lot drier. We
just block them with intestines and let them soak on ice water to soften.
Also
you can soak them in a glycerin solution this tends to soften the tissue for
good sectioning.....our techs swear by it.
lge
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