David,
	How long is your primary incubation period?  Are you using a Ventana
automatic stainer?
If so which one?
			C. Wright HT

-----Original Message-----
From: david moses [mailto:drmoses@erols.com]
Sent: Tuesday, May 11, 2004 11:34 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] Ventana's Confirm CD10?


I am working up Ventana's Confirm CD10 antibody.The
germinal centers of the normal tonsil are very pale, 
the follicular lymphoma is a little better and the kidney
staining is good. I'm using Trilogy(EDTA) in a Decloaker
for 4 min. at 120c, then a 10 min. cool down.  Any suggestions on how I can
improve the staining in my
lymphoid tissue? Thanks.
                                           Renee Boston-Moses HT

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